ECTS - Protein Biochemistry and Proteomics
Protein Biochemistry and Proteomics (CEAC515) Course Detail
| Course Name | Course Code | Season | Lecture Hours | Application Hours | Lab Hours | Credit | ECTS |
|---|---|---|---|---|---|---|---|
| Protein Biochemistry and Proteomics | CEAC515 | Area Elective | 3 | 0 | 0 | 3 | 5 |
| Pre-requisite Course(s) |
|---|
| N/A |
| Course Language | English |
|---|---|
| Course Type | Technical Elective Courses |
| Course Level | Ph.D. |
| Mode of Delivery | Face To Face |
| Learning and Teaching Strategies | Lecture, Demonstration, Question and Answer. |
| Course Lecturer(s) |
|
| Course Objectives | To identify the integration of cellular components and biochemical reactions and to explore proteomics tools to investigate protein levels,localization and function. |
| Course Learning Outcomes |
The students who succeeded in this course;
|
| Course Content | Protein concentration calculation, electrephoresis gels; SDS gels, native gels, staining gels, precipitation and concentration of proteins, blotting and protein staining on blots, immunostaining, proteomics principles, 2D electrophoresis, mass spectroscopy of peptides and proteins, mass spectrometers. |
Weekly Subjects and Releated Preparation Studies
| Week | Subjects | Preparation |
|---|---|---|
| 1 | Amino acids and protein structure | |
| 2 | Protein purification methods | |
| 3 | Protein analysis methods : Bradford method, Biuret Method | |
| 4 | Protein analysis methods; Lowry method, BCA method | |
| 5 | Electrophoresis, SDS gels, staining gels. | |
| 6 | MIDTERM I | |
| 7 | Blotting techniques and protein stainings on blotting | |
| 8 | Immunostaining methods | |
| 9 | Autoradiography of gels and blots | |
| 10 | Ligand binding and radioactive ligand marking | |
| 11 | MIDTERM II | |
| 12 | Proteomics principles and their applications | |
| 13 | Mass spectrophotometers for peptides and proteins | |
| 14 | The working principles of mass spectrophotometer and it is use | |
| 15 | Microsequencing; Edman Degredation, carboxyterminal sequencing, ladder sequencing of peptides | |
| 16 | FINAL EXAM |
Sources
Evaluation System
| Requirements | Number | Percentage of Grade |
|---|---|---|
| Attendance/Participation | - | - |
| Laboratory | - | - |
| Application | - | - |
| Field Work | - | - |
| Special Course Internship | - | - |
| Quizzes/Studio Critics | - | - |
| Homework Assignments | 2 | 15 |
| Presentation | - | - |
| Project | - | - |
| Report | - | - |
| Seminar | - | - |
| Midterms Exams/Midterms Jury | 2 | 50 |
| Final Exam/Final Jury | 1 | 35 |
| Toplam | 5 | 100 |
| Percentage of Semester Work | |
|---|---|
| Percentage of Final Work | 100 |
| Total | 100 |
Course Category
| Core Courses | |
|---|---|
| Major Area Courses | X |
| Supportive Courses | |
| Media and Managment Skills Courses | |
| Transferable Skill Courses |
The Relation Between Course Learning Competencies and Program Qualifications
| # | Program Qualifications / Competencies | Level of Contribution | ||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | ||
| 1 | An ability to access, analyze and evaluate the knowledge in chemistry and comprehend the literature. | X | ||||
| 2 | An ability to define an advanced scientific problem in the required field and finding an alternative for the solution. | X | ||||
| 3 | An ability to design and conduct scientific and technological experiments in the lab- and pilot-scale, and to analyze and interpret their results. | X | ||||
| 4 | An ability to perform independent research. | X | ||||
| 5 | An ability to give a presentation at national and international scientific conferences and to publish scientific publications in international journals. | X | ||||
| 6 | An ability to achieve the necessary knowledge to follow current developments in science and technology, and do scientific research or developing projects in the field of chemistry. | X | ||||
| 7 | An ability to work in a multi-disciplinary environment and to work as a part of a team. | X | ||||
| 8 | An understanding of the professional and occupational responsibilities. Awareness and responsibility about professional, legal, ethical and social issues in the required field. | X | ||||
ECTS/Workload Table
| Activities | Number | Duration (Hours) | Total Workload |
|---|---|---|---|
| Course Hours (Including Exam Week: 16 x Total Hours) | |||
| Laboratory | |||
| Application | |||
| Special Course Internship | |||
| Field Work | |||
| Study Hours Out of Class | 3 | 10 | 30 |
| Presentation/Seminar Prepration | |||
| Project | |||
| Report | |||
| Homework Assignments | 2 | 4 | 8 |
| Quizzes/Studio Critics | |||
| Prepration of Midterm Exams/Midterm Jury | 2 | 10 | 20 |
| Prepration of Final Exams/Final Jury | 1 | 20 | 20 |
| Total Workload | 78 | ||