ECTS - Cell Culture Techniques
Cell Culture Techniques (CEAC514) Course Detail
| Course Name | Course Code | Season | Lecture Hours | Application Hours | Lab Hours | Credit | ECTS |
|---|---|---|---|---|---|---|---|
| Cell Culture Techniques | CEAC514 | Area Elective | 3 | 0 | 0 | 3 | 5 |
| Pre-requisite Course(s) |
|---|
| N/A |
| Course Language | English |
|---|---|
| Course Type | Technical Elective Courses |
| Course Level | Ph.D. |
| Mode of Delivery | Face To Face |
| Learning and Teaching Strategies | Lecture, Demonstration, Question and Answer. |
| Course Lecturer(s) |
|
| Course Objectives | To provide knowledge about animal cell culture which has become an important and essential laboratory technique for the study of biochemical, physiological and pharmacological processes. |
| Course Learning Outcomes |
The students who succeeded in this course;
|
| Course Content | Basic cell culture techniques for bioscience research and commercial applications. introduction to cell cultivation methods, introduction to proper use of biological safety cabinet,sterile technique, cell enumaration and media preparartion,primary cultures,cultivation of cell lines, detection of contamination,cryopreservation,transfection,and an |
Weekly Subjects and Releated Preparation Studies
| Week | Subjects | Preparation |
|---|---|---|
| 1 | Historical Background in the advancement of TCC Technology. Basic information on cell: cell membrane and its function, organelles, general types of tissues of human origin | |
| 2 | Tissue Cell Culture Technology: Comparing organ, tissue and cell cultures | |
| 3 | Basics for primary and secondary cell cultures | |
| 4 | TCC techniques: Basic aseptic rules | |
| 5 | TCC techniques: Basic instrumantation | |
| 6 | TCC techniques: Basic labwares | |
| 7 | TCC techniques: 4.Cell line selection and supplementary media | |
| 8 | TCC techniques: 5.Seeding of cells(inoculation), passaging, harvesting of cells and cryopreservation | |
| 9 | Midterm | |
| 10 | TCC techniques: 6. Calculation of Growth rate in terms of growth curve and duplication time | |
| 11 | TCC techniques: 7. Cell viability and cell density calculations | |
| 12 | TCC techniques: 8. Basics for preparing suspension culture of cell lines from frozen stocks | |
| 13 | Midterm | |
| 14 | TCC techniques: 9. Basics for preparing adherent culture of cell lines from frozen stocks | |
| 15 | Biomedical and Industrial approach to TCC Technology | |
| 16 | Final |
Sources
Evaluation System
| Requirements | Number | Percentage of Grade |
|---|---|---|
| Attendance/Participation | - | - |
| Laboratory | - | - |
| Application | - | - |
| Field Work | - | - |
| Special Course Internship | - | - |
| Quizzes/Studio Critics | - | - |
| Homework Assignments | - | - |
| Presentation | - | - |
| Project | - | - |
| Report | - | - |
| Seminar | - | - |
| Midterms Exams/Midterms Jury | 2 | 25 |
| Final Exam/Final Jury | 1 | 35 |
| Toplam | 3 | 60 |
| Percentage of Semester Work | |
|---|---|
| Percentage of Final Work | 100 |
| Total | 100 |
Course Category
| Core Courses | |
|---|---|
| Major Area Courses | X |
| Supportive Courses | |
| Media and Managment Skills Courses | |
| Transferable Skill Courses |
The Relation Between Course Learning Competencies and Program Qualifications
| # | Program Qualifications / Competencies | Level of Contribution | ||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | ||
| 1 | An ability to access, analyze and evaluate the knowledge in chemistry and comprehend the literature. | X | ||||
| 2 | An ability to define an advanced scientific problem in the required field and finding an alternative for the solution. | X | ||||
| 3 | An ability to design and conduct scientific and technological experiments in the lab- and pilot-scale, and to analyze and interpret their results. | X | ||||
| 4 | An ability to perform independent research. | X | ||||
| 5 | An ability to give a presentation at national and international scientific conferences and to publish scientific publications in international journals. | X | ||||
| 6 | An ability to achieve the necessary knowledge to follow current developments in science and technology, and do scientific research or developing projects in the field of chemistry. | X | ||||
| 7 | An ability to work in a multi-disciplinary environment and to work as a part of a team. | X | ||||
| 8 | An understanding of the professional and occupational responsibilities. Awareness and responsibility about professional, legal, ethical and social issues in the required field. | X | ||||
ECTS/Workload Table
| Activities | Number | Duration (Hours) | Total Workload |
|---|---|---|---|
| Course Hours (Including Exam Week: 16 x Total Hours) | 14 | 3 | 42 |
| Laboratory | |||
| Application | |||
| Special Course Internship | |||
| Field Work | |||
| Study Hours Out of Class | 1 | 12 | 12 |
| Presentation/Seminar Prepration | |||
| Project | |||
| Report | |||
| Homework Assignments | |||
| Quizzes/Studio Critics | |||
| Prepration of Midterm Exams/Midterm Jury | 2 | 12 | 24 |
| Prepration of Final Exams/Final Jury | 1 | 36 | 36 |
| Total Workload | 114 | ||